Polymerase Mu Is a DNA-Directed DNA/RNA Polymerase
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چکیده
منابع مشابه
Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase.
The creation of novel enzymatic function is of great interest, but remains a challenge because of the large sequence space of proteins. We have developed an activity-based selection method to evolve DNA polymerases with RNA polymerase activity. The Stoffel fragment (SF) of Thermus aquaticus DNA polymerase I is displayed on a filamentous phage by fusing it to a pIII coat protein, and the substra...
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One of the most enigmat ic results of studies on the enzymic synthesis of D N A is tha t the polymerase is consistently found in homogena te fractions generally designated as cytoplasmic 0 4 ) . Since an unequivocal answer to the question of intracellular location of the enzyme seemed quite impossible from homogenate studies, we have taken a direct cytological approach to the problem. T he expe...
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chromatin-associated DNA-directed DNA polymerase activity, and the soluble RNase-sensitive endogenous DNA polymerase activity were examined in two fibroblast cell lines (WI-38 and WI-38VA13), in thirteen lymphoid cell lines originated from normal persons or from acute lymphocytic leukemia, acute myelocytic leukemia, Burkitt lymphoma, or multiple myeloma patients, and also in cells obtained dire...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 2003
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.23.7.2309-2315.2003